Cell adhesion and osteogenic differentiation on three‐dimensional pillar surfaces
Identifieur interne : 001625 ( Main/Exploration ); précédent : 001624; suivant : 001626Cell adhesion and osteogenic differentiation on three‐dimensional pillar surfaces
Auteurs : Emilia Kaivosoja [Finlande] ; Pia Suvanto [Finlande] ; Gonçalo Barreto [Finlande] ; Susanna Aura [Finlande] ; Antti Soininen [Finlande] ; Sami Franssila [Finlande] ; Yrjö T. Konttinen [Finlande]Source :
- Journal of Biomedical Materials Research Part A [ 1549-3296 ] ; 2013-03.
English descriptors
- Teeft :
- Aalto university, Actin cytoskeleton, Adhesion, Alkaline phosphatase, Biomater appl, Biomaterial surfaces, Biomed mater, Biomedical materials research, Bone nodule formation, Bone nodules, Cell adhesion, Cell culture, Cell density, Cell fate, Cell growth, Cell monolayer, Cell shape, Cells spread, Cluster formation, Culture medium, Cultured, Curved solenoid, Cytoskeletal, Cytoskeletal organization, Cytoskeletal tension, Cytoskeleton, Dh2o, Diamond relat mater, Different heights, Different results, Differentiation markers, Extracellular matrix, Finland, Ground level, Height differences, Helsinki, Human mesenchymal, Implant, Interspaces, Kaivosoja, Konttinen, Mater, Mesenchymal, Mesenchymal cell growth supplement, Monoclonal mouse, Msc, Nodule, Nuclear stain, Online, Online issue, Original article, Original article figure, Ormocompv, Ormocompv layer, Osteoblastic cells, Osteogenesis, Osteogenic, Osteogenic differentiation, Osteopontin, Other areas, Oxford instruments, Petri dishes, Physical properties, Pillar, Pillar area, Pillar structures, Pillar surface, Pillar surfaces, Pillar surfaces kaivosoja, Planar, Planar substrates, Planar surface, Planar surfaces, Proc natl acad, Protein adsorption, Silicon, Strong staining, Tio2, Tio2 pillars, Weak staining, Wiley periodicals.
Abstract
We hypothesized that when compared with conventional two‐dimensional (2D) cultures, substrates containing 3D micropillars would allow cells to grow at levels, activating their cytoskeleton to promote osteogenesis. Fibroblasts, osteoblast‐like cells, and mesenchymal stem cells (MSCs) were studied. Planar substrates were compared with 200‐nm‐, 5‐μm‐, and 20‐μm‐high pillars of Ormocomp®, Si, diamond‐like carbon, or TiO2. Scanning electron microscopy and staining of actin cytoskeleton showed 7.5‐h adhesion to pillar edges and 5‐day stretching between adhesion contacts > 100‐μm distances of fibroblast and MSC in 3D networks, whereas SaOS‐2 cells adhered flatly and individually on horizontal and vertical surfaces. ERK and ROCK immunostaining at 14 and 21 days confirmed activation of the cytoskeleton. In contrast to expectations, success to induce osteogenesis was dominated by the cytocompatibility of the substrate over the 3D structure. This was shown using early alkaline phosphatase, intermediate osteopontin, and late mineralization markers, together with bone nodule formation, which were seen in planar substrates and low‐profile TiO2 pillars, but were poor in the 20‐μm landscape. The lack of intercellular contacts seems to halt the osteogenesis‐promoting effects of cytoskeletal organization and tension described earlier. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 842–852, 2013.
Url:
DOI: 10.1002/jbm.a.34378
Affiliations:
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Konttinen</name><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>ORTON Research Institute of the ORTON Orthopaedic Hospital, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>COXA Hospital for Joint Replacement, Tampere</wicri:regionArea><wicri:noRegion>Tampere</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Finlande</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Finlande</country><wicri:regionArea>Correspondence address: Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Helsinki</wicri:regionArea><wicri:noRegion>Helsinki</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part A</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A</title><idno type="ISSN">1549-3296</idno><idno type="eISSN">1552-4965</idno><imprint><biblScope unit="vol">101A</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="842">842</biblScope><biblScope unit="page" to="852">852</biblScope><biblScope unit="page-count">11</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-03">2013-03</date></imprint><idno type="ISSN">1549-3296</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1549-3296</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aalto university</term><term>Actin cytoskeleton</term><term>Adhesion</term><term>Alkaline phosphatase</term><term>Biomater appl</term><term>Biomaterial surfaces</term><term>Biomed mater</term><term>Biomedical materials research</term><term>Bone nodule formation</term><term>Bone nodules</term><term>Cell adhesion</term><term>Cell culture</term><term>Cell density</term><term>Cell fate</term><term>Cell growth</term><term>Cell monolayer</term><term>Cell shape</term><term>Cells spread</term><term>Cluster formation</term><term>Culture medium</term><term>Cultured</term><term>Curved solenoid</term><term>Cytoskeletal</term><term>Cytoskeletal organization</term><term>Cytoskeletal tension</term><term>Cytoskeleton</term><term>Dh2o</term><term>Diamond relat mater</term><term>Different heights</term><term>Different results</term><term>Differentiation markers</term><term>Extracellular matrix</term><term>Finland</term><term>Ground level</term><term>Height differences</term><term>Helsinki</term><term>Human mesenchymal</term><term>Implant</term><term>Interspaces</term><term>Kaivosoja</term><term>Konttinen</term><term>Mater</term><term>Mesenchymal</term><term>Mesenchymal cell growth supplement</term><term>Monoclonal mouse</term><term>Msc</term><term>Nodule</term><term>Nuclear stain</term><term>Online</term><term>Online issue</term><term>Original article</term><term>Original article figure</term><term>Ormocompv</term><term>Ormocompv layer</term><term>Osteoblastic cells</term><term>Osteogenesis</term><term>Osteogenic</term><term>Osteogenic differentiation</term><term>Osteopontin</term><term>Other areas</term><term>Oxford instruments</term><term>Petri dishes</term><term>Physical properties</term><term>Pillar</term><term>Pillar area</term><term>Pillar structures</term><term>Pillar surface</term><term>Pillar surfaces</term><term>Pillar surfaces kaivosoja</term><term>Planar</term><term>Planar substrates</term><term>Planar surface</term><term>Planar surfaces</term><term>Proc natl acad</term><term>Protein adsorption</term><term>Silicon</term><term>Strong staining</term><term>Tio2</term><term>Tio2 pillars</term><term>Weak staining</term><term>Wiley periodicals</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We hypothesized that when compared with conventional two‐dimensional (2D) cultures, substrates containing 3D micropillars would allow cells to grow at levels, activating their cytoskeleton to promote osteogenesis. Fibroblasts, osteoblast‐like cells, and mesenchymal stem cells (MSCs) were studied. Planar substrates were compared with 200‐nm‐, 5‐μm‐, and 20‐μm‐high pillars of Ormocomp®, Si, diamond‐like carbon, or TiO2. Scanning electron microscopy and staining of actin cytoskeleton showed 7.5‐h adhesion to pillar edges and 5‐day stretching between adhesion contacts > 100‐μm distances of fibroblast and MSC in 3D networks, whereas SaOS‐2 cells adhered flatly and individually on horizontal and vertical surfaces. ERK and ROCK immunostaining at 14 and 21 days confirmed activation of the cytoskeleton. In contrast to expectations, success to induce osteogenesis was dominated by the cytocompatibility of the substrate over the 3D structure. This was shown using early alkaline phosphatase, intermediate osteopontin, and late mineralization markers, together with bone nodule formation, which were seen in planar substrates and low‐profile TiO2 pillars, but were poor in the 20‐μm landscape. The lack of intercellular contacts seems to halt the osteogenesis‐promoting effects of cytoskeletal organization and tension described earlier. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 842–852, 2013.</div></front></TEI><affiliations><list><country><li>Finlande</li></country><region><li>Uusimaa</li></region><settlement><li>Espoo</li></settlement><orgName><li>Université Aalto</li></orgName></list><tree><country name="Finlande"><noRegion><name sortKey="Kaivosoja, Emilia" sort="Kaivosoja, Emilia" uniqKey="Kaivosoja E" first="Emilia" last="Kaivosoja">Emilia Kaivosoja</name></noRegion><name sortKey="Aura, Susanna" sort="Aura, Susanna" uniqKey="Aura S" first="Susanna" last="Aura">Susanna Aura</name><name sortKey="Barreto, Goncalo" sort="Barreto, Goncalo" uniqKey="Barreto G" first="Gonçalo" last="Barreto">Gonçalo Barreto</name><name sortKey="Franssila, Sami" sort="Franssila, Sami" uniqKey="Franssila S" first="Sami" last="Franssila">Sami Franssila</name><name sortKey="Kaivosoja, Emilia" sort="Kaivosoja, Emilia" uniqKey="Kaivosoja E" first="Emilia" last="Kaivosoja">Emilia Kaivosoja</name><name sortKey="Konttinen, Yrjo T" sort="Konttinen, Yrjo T" uniqKey="Konttinen Y" first="Yrjö T." last="Konttinen">Yrjö T. Konttinen</name><name sortKey="Konttinen, Yrjo T" sort="Konttinen, Yrjo T" uniqKey="Konttinen Y" first="Yrjö T." last="Konttinen">Yrjö T. Konttinen</name><name sortKey="Konttinen, Yrjo T" sort="Konttinen, Yrjo T" uniqKey="Konttinen Y" first="Yrjö T." last="Konttinen">Yrjö T. Konttinen</name><name sortKey="Konttinen, Yrjo T" sort="Konttinen, Yrjo T" uniqKey="Konttinen Y" first="Yrjö T." last="Konttinen">Yrjö T. Konttinen</name><name sortKey="Konttinen, Yrjo T" sort="Konttinen, Yrjo T" uniqKey="Konttinen Y" first="Yrjö T." last="Konttinen">Yrjö T. Konttinen</name><name sortKey="Soininen, Antti" sort="Soininen, Antti" uniqKey="Soininen A" first="Antti" last="Soininen">Antti Soininen</name><name sortKey="Suvanto, Pia" sort="Suvanto, Pia" uniqKey="Suvanto P" first="Pia" last="Suvanto">Pia Suvanto</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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